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cdna end 5 race analyses  (Five Prime)

 
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    Five Prime cdna end 5 race analyses
    (A) A schematic represents 5’ RACE analyses and provirus analyses. 293T cells were transfected with an HIV-1 clone plasmid (pNL4-3EGFP ΔenvΔnef WT or mutant plasmid) and pMISSION-VSV-G to produce the VSV-G-pseudotyped HIV-1. Genomic RNAs in the particles were purified and subsequently subjected to 5’ RACE. For provirus analyses, MT-4 cells were exposed with supernatant containing the VSV-G-pseudotyped virus. Genomic DNA containing HIV-1 provirus were purified from MT-4 cells. (B) Results of 5’ RACE analyses are shown. 5’RACE of purified RNA from the VSV-G-pseudotyped virus particles were done for the AAA-AAA mutant virus (n=34 clones). Nucleotides different from the input plasmid for producing particles ( and ) are highlighted as bold characters. Hyphens are used for forms not observed in the analyses. (C) HIV-1 reverse-transcriptase has been reported to have the ability to overcome mismatched 3’ termini between a template RNA and minus-strand strong-stop cDNA (-sscDNA). A schematic based on the knowledge represents predicted reverse-transcription processes to generate unexpected proviral sequences (AC A AAA; the region of the tract is highlighted with an underline) with the 4A form template RNA. Genomic RNAs, DNAs, acquired mutations and tRNAs are drawn in black, blue, red and green, respectively. (D-E) Results of 5’ RACE analyses are shown as described in . The analyses of purified RNA from the particles were done for the CCC-CCC (C; n=34 clones) or the TTT-TTT mutant virus (D; n=34 clones).
    Cdna End 5 Race Analyses, supplied by Five Prime, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+end+5+race+analyses/bio_rxiv__64898__2026__04__24__720579-5-4-0?v=Five+Prime
    Average 86 stars, based on 1 article reviews
    cdna end 5 race analyses - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "The strictly conserved GGG-tracts in the 5’ and 3’ long terminal repeat of HIV-1 are critical to control multiple steps of HIV-1 replication to prevent acquisition of unwanted mutations in the region"

    Article Title: The strictly conserved GGG-tracts in the 5’ and 3’ long terminal repeat of HIV-1 are critical to control multiple steps of HIV-1 replication to prevent acquisition of unwanted mutations in the region

    Journal: bioRxiv

    doi: 10.64898/2026.04.24.720579

    (A) A schematic represents 5’ RACE analyses and provirus analyses. 293T cells were transfected with an HIV-1 clone plasmid (pNL4-3EGFP ΔenvΔnef WT or mutant plasmid) and pMISSION-VSV-G to produce the VSV-G-pseudotyped HIV-1. Genomic RNAs in the particles were purified and subsequently subjected to 5’ RACE. For provirus analyses, MT-4 cells were exposed with supernatant containing the VSV-G-pseudotyped virus. Genomic DNA containing HIV-1 provirus were purified from MT-4 cells. (B) Results of 5’ RACE analyses are shown. 5’RACE of purified RNA from the VSV-G-pseudotyped virus particles were done for the AAA-AAA mutant virus (n=34 clones). Nucleotides different from the input plasmid for producing particles ( and ) are highlighted as bold characters. Hyphens are used for forms not observed in the analyses. (C) HIV-1 reverse-transcriptase has been reported to have the ability to overcome mismatched 3’ termini between a template RNA and minus-strand strong-stop cDNA (-sscDNA). A schematic based on the knowledge represents predicted reverse-transcription processes to generate unexpected proviral sequences (AC A AAA; the region of the tract is highlighted with an underline) with the 4A form template RNA. Genomic RNAs, DNAs, acquired mutations and tRNAs are drawn in black, blue, red and green, respectively. (D-E) Results of 5’ RACE analyses are shown as described in . The analyses of purified RNA from the particles were done for the CCC-CCC (C; n=34 clones) or the TTT-TTT mutant virus (D; n=34 clones).
    Figure Legend Snippet: (A) A schematic represents 5’ RACE analyses and provirus analyses. 293T cells were transfected with an HIV-1 clone plasmid (pNL4-3EGFP ΔenvΔnef WT or mutant plasmid) and pMISSION-VSV-G to produce the VSV-G-pseudotyped HIV-1. Genomic RNAs in the particles were purified and subsequently subjected to 5’ RACE. For provirus analyses, MT-4 cells were exposed with supernatant containing the VSV-G-pseudotyped virus. Genomic DNA containing HIV-1 provirus were purified from MT-4 cells. (B) Results of 5’ RACE analyses are shown. 5’RACE of purified RNA from the VSV-G-pseudotyped virus particles were done for the AAA-AAA mutant virus (n=34 clones). Nucleotides different from the input plasmid for producing particles ( and ) are highlighted as bold characters. Hyphens are used for forms not observed in the analyses. (C) HIV-1 reverse-transcriptase has been reported to have the ability to overcome mismatched 3’ termini between a template RNA and minus-strand strong-stop cDNA (-sscDNA). A schematic based on the knowledge represents predicted reverse-transcription processes to generate unexpected proviral sequences (AC A AAA; the region of the tract is highlighted with an underline) with the 4A form template RNA. Genomic RNAs, DNAs, acquired mutations and tRNAs are drawn in black, blue, red and green, respectively. (D-E) Results of 5’ RACE analyses are shown as described in . The analyses of purified RNA from the particles were done for the CCC-CCC (C; n=34 clones) or the TTT-TTT mutant virus (D; n=34 clones).

    Techniques Used: Transfection, Plasmid Preparation, Mutagenesis, Purification, Virus, Clone Assay, Reverse Transcription

    (A) Nucleotide sequences of the CCC-AAA, CCC-GGG and CCC-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the CCC-AAA (left panels; n=38 clones), the CCC-GGG (center panels; n=40 clones) and the CCC-TTT mutant virus (right panels; n=39 clones).
    Figure Legend Snippet: (A) Nucleotide sequences of the CCC-AAA, CCC-GGG and CCC-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the CCC-AAA (left panels; n=38 clones), the CCC-GGG (center panels; n=40 clones) and the CCC-TTT mutant virus (right panels; n=39 clones).

    Techniques Used: Purification, Clone Assay, Mutagenesis, Virus

    (A) Nucleotide sequences of the GGG-AAA, GGG-CCC and GGG-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B-D) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the GGG-AAA (B; n=43 clones), the GGG-CCC (C; n=40 clones) and the GGG-TTT mutant virus (D; n=38 clones).
    Figure Legend Snippet: (A) Nucleotide sequences of the GGG-AAA, GGG-CCC and GGG-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B-D) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the GGG-AAA (B; n=43 clones), the GGG-CCC (C; n=40 clones) and the GGG-TTT mutant virus (D; n=38 clones).

    Techniques Used: Purification, Clone Assay, Mutagenesis, Virus



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    (A) A schematic represents 5’ RACE analyses and provirus analyses. 293T cells were transfected with an HIV-1 clone plasmid (pNL4-3EGFP ΔenvΔnef WT or mutant plasmid) and pMISSION-VSV-G to produce the VSV-G-pseudotyped HIV-1. Genomic RNAs in the particles were purified and subsequently subjected to 5’ RACE. For provirus analyses, MT-4 cells were exposed with supernatant containing the VSV-G-pseudotyped virus. Genomic DNA containing HIV-1 provirus were purified from MT-4 cells. (B) Results of 5’ RACE analyses are shown. 5’RACE of purified RNA from the VSV-G-pseudotyped virus particles were done for the AAA-AAA mutant virus (n=34 clones). Nucleotides different from the input plasmid for producing particles ( and ) are highlighted as bold characters. Hyphens are used for forms not observed in the analyses. (C) HIV-1 reverse-transcriptase has been reported to have the ability to overcome mismatched 3’ termini between a template RNA and minus-strand strong-stop cDNA (-sscDNA). A schematic based on the knowledge represents predicted reverse-transcription processes to generate unexpected proviral sequences (AC A AAA; the region of the tract is highlighted with an underline) with the 4A form template RNA. Genomic RNAs, DNAs, acquired mutations and tRNAs are drawn in black, blue, red and green, respectively. (D-E) Results of 5’ RACE analyses are shown as described in . The analyses of purified RNA from the particles were done for the CCC-CCC (C; n=34 clones) or the TTT-TTT mutant virus (D; n=34 clones).
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    Image Search Results


    (A) A schematic represents 5’ RACE analyses and provirus analyses. 293T cells were transfected with an HIV-1 clone plasmid (pNL4-3EGFP ΔenvΔnef WT or mutant plasmid) and pMISSION-VSV-G to produce the VSV-G-pseudotyped HIV-1. Genomic RNAs in the particles were purified and subsequently subjected to 5’ RACE. For provirus analyses, MT-4 cells were exposed with supernatant containing the VSV-G-pseudotyped virus. Genomic DNA containing HIV-1 provirus were purified from MT-4 cells. (B) Results of 5’ RACE analyses are shown. 5’RACE of purified RNA from the VSV-G-pseudotyped virus particles were done for the AAA-AAA mutant virus (n=34 clones). Nucleotides different from the input plasmid for producing particles ( and ) are highlighted as bold characters. Hyphens are used for forms not observed in the analyses. (C) HIV-1 reverse-transcriptase has been reported to have the ability to overcome mismatched 3’ termini between a template RNA and minus-strand strong-stop cDNA (-sscDNA). A schematic based on the knowledge represents predicted reverse-transcription processes to generate unexpected proviral sequences (AC A AAA; the region of the tract is highlighted with an underline) with the 4A form template RNA. Genomic RNAs, DNAs, acquired mutations and tRNAs are drawn in black, blue, red and green, respectively. (D-E) Results of 5’ RACE analyses are shown as described in . The analyses of purified RNA from the particles were done for the CCC-CCC (C; n=34 clones) or the TTT-TTT mutant virus (D; n=34 clones).

    Journal: bioRxiv

    Article Title: The strictly conserved GGG-tracts in the 5’ and 3’ long terminal repeat of HIV-1 are critical to control multiple steps of HIV-1 replication to prevent acquisition of unwanted mutations in the region

    doi: 10.64898/2026.04.24.720579

    Figure Lengend Snippet: (A) A schematic represents 5’ RACE analyses and provirus analyses. 293T cells were transfected with an HIV-1 clone plasmid (pNL4-3EGFP ΔenvΔnef WT or mutant plasmid) and pMISSION-VSV-G to produce the VSV-G-pseudotyped HIV-1. Genomic RNAs in the particles were purified and subsequently subjected to 5’ RACE. For provirus analyses, MT-4 cells were exposed with supernatant containing the VSV-G-pseudotyped virus. Genomic DNA containing HIV-1 provirus were purified from MT-4 cells. (B) Results of 5’ RACE analyses are shown. 5’RACE of purified RNA from the VSV-G-pseudotyped virus particles were done for the AAA-AAA mutant virus (n=34 clones). Nucleotides different from the input plasmid for producing particles ( and ) are highlighted as bold characters. Hyphens are used for forms not observed in the analyses. (C) HIV-1 reverse-transcriptase has been reported to have the ability to overcome mismatched 3’ termini between a template RNA and minus-strand strong-stop cDNA (-sscDNA). A schematic based on the knowledge represents predicted reverse-transcription processes to generate unexpected proviral sequences (AC A AAA; the region of the tract is highlighted with an underline) with the 4A form template RNA. Genomic RNAs, DNAs, acquired mutations and tRNAs are drawn in black, blue, red and green, respectively. (D-E) Results of 5’ RACE analyses are shown as described in . The analyses of purified RNA from the particles were done for the CCC-CCC (C; n=34 clones) or the TTT-TTT mutant virus (D; n=34 clones).

    Article Snippet: Five-prime rapid amplification of cDNA end (5’ RACE) analyses of RNAs purified from mutant virus particles revealed multiple RNA variants with 5’ terminal sequences differing from the plasmid used for producing the particles.

    Techniques: Transfection, Plasmid Preparation, Mutagenesis, Purification, Virus, Clone Assay, Reverse Transcription

    (A) Nucleotide sequences of the CCC-AAA, CCC-GGG and CCC-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the CCC-AAA (left panels; n=38 clones), the CCC-GGG (center panels; n=40 clones) and the CCC-TTT mutant virus (right panels; n=39 clones).

    Journal: bioRxiv

    Article Title: The strictly conserved GGG-tracts in the 5’ and 3’ long terminal repeat of HIV-1 are critical to control multiple steps of HIV-1 replication to prevent acquisition of unwanted mutations in the region

    doi: 10.64898/2026.04.24.720579

    Figure Lengend Snippet: (A) Nucleotide sequences of the CCC-AAA, CCC-GGG and CCC-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the CCC-AAA (left panels; n=38 clones), the CCC-GGG (center panels; n=40 clones) and the CCC-TTT mutant virus (right panels; n=39 clones).

    Article Snippet: Five-prime rapid amplification of cDNA end (5’ RACE) analyses of RNAs purified from mutant virus particles revealed multiple RNA variants with 5’ terminal sequences differing from the plasmid used for producing the particles.

    Techniques: Purification, Clone Assay, Mutagenesis, Virus

    (A) Nucleotide sequences of the GGG-AAA, GGG-CCC and GGG-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B-D) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the GGG-AAA (B; n=43 clones), the GGG-CCC (C; n=40 clones) and the GGG-TTT mutant virus (D; n=38 clones).

    Journal: bioRxiv

    Article Title: The strictly conserved GGG-tracts in the 5’ and 3’ long terminal repeat of HIV-1 are critical to control multiple steps of HIV-1 replication to prevent acquisition of unwanted mutations in the region

    doi: 10.64898/2026.04.24.720579

    Figure Lengend Snippet: (A) Nucleotide sequences of the GGG-AAA, GGG-CCC and GGG-TTT mutants of NL4-3EGFP ΔenvΔnef are shown as described in . (B-D) Results of 5’ RACE analyses are shown as described in . 5’ RACE analyses of purified RNA from the particles were done as described in for the GGG-AAA (B; n=43 clones), the GGG-CCC (C; n=40 clones) and the GGG-TTT mutant virus (D; n=38 clones).

    Article Snippet: Five-prime rapid amplification of cDNA end (5’ RACE) analyses of RNAs purified from mutant virus particles revealed multiple RNA variants with 5’ terminal sequences differing from the plasmid used for producing the particles.

    Techniques: Purification, Clone Assay, Mutagenesis, Virus